The second step in BH 4 synthesis, catalyzed by 6-pyruvoyltetrahydropterin synthase (PTPS), involves the Zn 2+ and Mg 2+ -dependent rearrangement of 7,8-dihydroneopterin triphosphate, with concommitant loss of the triphosphate, to 6-pyruvoyltetrahydropterin
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In research settings, this allows investigators to observe discrete signaling events and timing-dependent assay responses in controlled experimental models
Our initial attempt to create a plasmid based on the biotin ligase BirA backbone (Eric Campeau, Addgene plasmid #26624) was unsuccessful